Chemistry: analytical and immunological testing – Biological cellular material tested
Patent
1991-11-07
1994-03-29
Housel, James C.
Chemistry: analytical and immunological testing
Biological cellular material tested
435 6, 436 10, 436172, G01N 130
Patent
active
052984265
ABSTRACT:
A method which comprises using a specified dye taken up by erythrocytic nucleated cells so that their nuclei are stained and can be readily differentiated from other cells by measurement with a flow cytometer is disclosed. The method relies basically upon flow cytometry by two-step staining that uses a first fluid that is an acidic hypotensive fluorescent dye solution and a second fluid that is a solution that changes the osmorality and pH of the first fluid. The first fluid further contains a fluorescent dye that diffuses into erythroblasts to specifically stain their nuclei, and a group of erythroblasts is separated from other cell groups on the two-dimensional plot constructed with the flow cytometer, whereby the results of erythroblast differentiation are computed.
REFERENCES:
patent: 3916205 (1975-10-01), Kleinerman
patent: 4485175 (1984-11-01), Ledis et al.
patent: 4499183 (1985-02-01), Sujansky et al.
patent: 4751188 (1988-06-01), Valet
patent: 4978624 (1990-12-01), Cremins et al.
patent: 5039613 (1991-08-01), Matsuda et al.
patent: 5047321 (1991-09-01), Loken et al.
Inami Keiichi
Matsumoto Hideaki
Housel James C.
TOA Medical Electronics Co., Ltd.
Torres Ramon
LandOfFree
Method of differentiating erythroblasts from other cells by flow does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method of differentiating erythroblasts from other cells by flow, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method of differentiating erythroblasts from other cells by flow will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-791042