Method of differentiating between ulcerative colitis and...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

Reexamination Certificate

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C536S023100, C536S023500, C536S024310

Reexamination Certificate

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07378239

ABSTRACT:
The differentiation between ulcerative colitis and Crohn's disease is made possible by a multi-gene approach where gene expression profiles in biopsy samples obtained from inflamed, and optionally also non-inflamed, areas in the intestines of a patient are studied.

REFERENCES:
patent: 2003/0158254 (2003-08-01), Zerangue et al.
Lawrence et al. Ulcerative colitis and Crohn's disease: distinctive gene expression profiles and novel susceptibility candidte genes. Human Molecular Genetics, vol. 10, No. 5, pp. 445-456, 2001.
Online Mendelian Inheritance in Man, OMIM (TM). Johns Hopkins University, Baltimore, MD. MIM No.: 607178: Nov. 22, 2002: , World Wide Web URL: http://www.ncbi.nlm.nih.gov/omim/.
Anderle et al. Changes in the transcriptional profile of transporters in the intestine along the anterior-posterior and crypt-villus axes. BMC Genomics, vol. 6, No. 1, p. 69 (pp. 1/17-17-17), May 2005.
GenBank Accession No. XM—010112.1, GI: 11419739, publicly available Nov. 2000.
Haila et al. SLC26A2 (diastrophic dysplasia sulfate transporter) is expressed in developing and mature cartilage but also in other tissues and cell types. The Journal of Histochemistry & Cytochemistry, vol. 49, No. 8, pp. 973-982, Aug. 2001.
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Buck et al. Design strategies and performance of custom DNA sequencing primers. Biotechniques, vol. 27, No. 3, pp. 528-536, Sep. 1999.
A. Imada et al., “Coordinate Upregulation of Interleukin-8 and Growth-Related Gene Product-60 is Present in the Colonic Mucosa of Inflammatory Bowel Disease,” Scand. J. Gastroenterol., V. 36, 2001, pp. 854-864.
Ken J. Newell et al., “Matrilysin (Matrix Metalloproteinase-7) Expression in Ulcerative Colitis-Related Tumorigenesis,” Molecular Carcinogenesis, V. 34, 2002, pp. 59-63.
Kim L. Isaacs et al., “Cytokine Messenger RNA Profiles in Inflammatory Bowel Disease Mucosa Detected by Polymerase Chain Reaction Amplification,” Gastroenterology, V. 103, 1992, pp. 1587-1595.
J.C. Hartupee et al., “Isolation and Characterization of a cDNA Encoding a Novel Member of the Human Regenerating Protein Family: Reg IV1,” Biochimica et Biophysica Acta, V. 1518, 2001, pp. 287-293.
Keiki Matsuno et al., “The Expression of Matrix Metalloproteinase Matrilysin Indicates the Degree of Inflammation in Ulcerative Colitis,” Journal of Gastroenterology, V. 38, 2003, pp. 348-354.

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