Method of differential assay for .alpha.-amylase isozymes and a

Details Method of differential assay for .alpha.-amylase isozymes and a Method of differential assay for .alpha.-amylase isozymes and a

1992-05-08

1994-09-27

Nucker, Christine M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving hydrolase

435 18, 435810, C12Q 140

Patent

active

053506782

ABSTRACT:
Differential assay of human pancreatic .alpha.-amylase and human salivary .alpha.-amylase can be made extremely easily with good accuracy using a reducing-end modified maltooligosaccharide derivative as a substrate, by acting coupled enzymes having different substrate specificities on the degradation products formed by the hydrolytic action of .alpha.-amylases and measuring the resulting reaction products.

REFERENCES:
Omichi et al. Kagaku (Kyoto) 1986, 41 (2), pp. 138-139 "Fluorogenic Substrates of Human .alpha.-Amylase. Discrimination Between Pancreatic and Salivary .alpha. Amylases".
Clin. Chim. Acta, 54, (1974) pp. 137-144.
Clin. Chem. 18, No. 12 (1972) pp. 1493-1496.
J. Biochemistry, 97, (1985) pp. 1357-1362.
Clin. Chem., 23 560 (1977).
Chemical Abstracts, vol. 106, No. 11, Mar. 16, 1987, p. 252, Col. 1.
Chemical Abstracts, vol. 101, No. 11, Oct. 10, 1984, p. 252, Col. 1.
Patent Abstracts of Japan, vol. 9, No. 331 (C-321 [2054], Dec. 25, 1985.
Clinical Chemistry, vol. 28, No. 7, Jul. 1982, pp. 1525-1527.

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