Method of diagnosing predisposition for ulcerative colitis in Je

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

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435 691, 435 911, 4351723, 435333, 530350, 935 18, 935 78, 536 2433, 536 231, C12Q 168, C12P 2106, C07K 100, C07H 2104

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059423900

ABSTRACT:
A novel, differential association between allele 2 of the variable number of tandem repeats polymorphism at intron 2 of the human IL-1 receptor antagonist gene and ulcerative colitis in humans of Jewish ancestry has been discovered. In accordance with the present invention, there is provided methods of screening for ulcerative colitis in human subjects of Jewish ancestry comprising detecting the presence or absence of nucleic acid of the subject encoding allele 2 of the variable number of tandem repeats polymorphism at intron 2 of the human IL-1 receptor antagonist gene, wherein the presence of nucleic acid encoding allele 2 is indicative of ulcerative colitis. Kits useful for screening for ulcerative colitis in human subjects of Jewish ancestry are also provided.

REFERENCES:
Roth, M-P, et al., "Familial Empiric Risk Estimates of Inflammatory Bowel Disease in Ashkenazi Jews", Gastroenterology, 96:1016-1020, 1989.
Bioque, G., et al., "Further Evidence For a Genetic Association of Interleukin-1 Receptor Antagonist With Ulcerative Colitis In A Northern And A Mediterranean Population," Gastroenterology, 108(4):a783 (Apr. 1995).
Duerr, R.H., et al., "Association Between Ulcerative Colitis And A Polymorphism In Intron 2 Of The Interleukin-1 Receptor Antagonist Gene," Gastroenterology, 108(4):a812 (Apr. 1995).
Mansfield, J., et al., "Novel Genetic Association Between Ulcerative Colitis And The Anti-inflammatory Cytokine Interleukin-1 Receptor Antagonist," Gastroenterology, 106(3):637-642 (Mar. 1994).
Tountas, N.A., et al., "Genetic Association Between Allele 2 of IL-1 Receptor Antagonist (IL-1ra) And Ulcerative Colitis (UC) in a Los Angeles Based Hispanic Population," Gastroenterology, 108(4):a930 (Apr. 1995).
Tountas, N.A. et al., Hetrogenous Association Between Allele 2 of IL-1 Receptor Antagonist (IL-1ra) And Ulcerative Colitis (UC) In Jewish And Non-Jewish Populations J. Investigative Medicine, 44(1):a175 (Jan. 1996).
Casini-Raggi, V., et al., "Mucosal Imbalance of IL-1 and IL-1 Receptor Antagonist in Inflammatory Bowel Disease; A Novel Mechanism of Chronic Intestinal Inflammation." J. Immunol., :2434-2440 (1995).
Mansfield, J.C., et al., "Novel Genetic Association Between Ulcerative Colitis and the Anti-inflammatory Cytokine Interleukin-1 Receptor Antagonist." Gastroenterology, 106:637-642 (1994).
Tarlow, J.K., et al., "Polymorphism in Hhuman IL-1 Receptor Antagonist Gene Intron 2 is Caused by Variable Numbers of an 86-bp Tandem Repeat." Hum. Genet., 91:403-404 (1993).

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