Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving hydrolase
Patent
1996-11-14
2000-05-16
Gitomer, Ralph
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving hydrolase
435 74, C12Q 134
Patent
active
060635886
ABSTRACT:
This invention provides a method of diagnosing periodontal disease in a subject by detecting elevated concentrations of .beta.-glucuronidase in saliva from the subject. The concentration of .beta.-glucuronidase in the subject's saliva may be determined by adding to a sample of the saliva a substrate for .beta.-glucuronidase and measuring the amount of a product produced by the reaction of .beta.-glucuronidase on the substrate. Also, the concentration of .beta.-glucuronidase in the subject's saliva may be determined by adding to a sample of saliva a labeled antibody specific for .beta.-glucuronidase and measuring the amount of labeled antibody which complexes with .beta.-glucuronidase present in the saliva.
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Gitomer Ralph
The Trustees of Columbia University in the City of New York
White John P.
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