Method of diagnosing non-alcoholic steatohepatitis (nash)...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

Reexamination Certificate

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C435S007200, C435S007210, C436S518000, C436S169000

Reexamination Certificate

active

07314720

ABSTRACT:
The invention relates to a method of diagnosing non-alcoholic steatohepatitis (NASH) using molecular markers. The inventive method consists in detecting and quantifying, in vitro in a hepatic tissue sample, the levels of a protein which can be used as a NASH molecular marker and which is selected from apolipoprotein A1, sub-unit β of the mitochondrial ATPase, leukotriene A4 hydrolase, keratin 18, guanidine acetate N-methyltransferase, superoxide dismutase, albumin, antioxidant protein 2 (isoform 1), prohibitin 1, methionine adenosyl transferase, long-chain acyl CoA dehydrogenase, selenium binding protein, antioxidant protein 2 (isoform 2), and combinations of same. The invention further consists in comparing the results obtained with the normal values of said proteins in healthy hepatic tissue. Said method can be used to diagnose NASH and/or to assess a patient's potential risk of developing NASH.

REFERENCES:
patent: 2006/0084057 (2006-04-01), Rose et al.
Lonardo et al. Hepatology 2002 vol. 36, p. 514-515.
Lu, S.C., et al. “Methionine Adenosyltransferase 1A Knockout Mice are Predisposed to Liver Injury and Exhibit Increased Expression of Genes Involved in Proliferation”. PNAS,May 8, 2001, vol. 98, No. 10, p. 5560-5565.
Mato, J.M., et al. “S-Adenosylmethionine: A Control Switch That Regulates Liver Function” FASEB Journal, vol. 16, Jan. 2002, pp. 15-26.
Zatloukal, K., et al. “Alcoholic and Nonalcoholic Steatohepatitis” Rev. Esp. Patoi., vol. 32, No. 3, 1999, pp. 293-294.
Santamaria, E., et al. “Functional Proteomics of Nonalcoholic Steatohepatitis: Mitochondrial Proteins as Targets of S-adenosylmethionine” PNAS, vol. 100, No. 6, Mar. 18, 2003, pp. 3065-3070.
Sanyal, Arun J., AGA technical review on nonalcoholic fatty liver disease, Gastroenterology, Nov. 2002, pp. 1705-1725, vol. 123, No. 5.

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