Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism
Patent
1988-06-03
1991-09-24
Rosen, Sam
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving viable micro-organism
435 29, 435 31, 435805, C12Q 118
Patent
active
050513591
ABSTRACT:
An in situ method of determining the quality of a medium capable of suppong cellular growth comprises obtaining viable cells which are anchored to a substrate, determining the value of at least one characteristic of the cell which is proportional by a factor to the number of the cells present under conditions which preserve cell viability, calculating the number of cells present by multiplying the value of the characteristic by the factor, exposing the cells to the medium by in situ immersing the substrate with the anchored cells in the medium for a period of time and under conditions which preserve the viability of the cells and permit cell growth, withdrawiing the cells from the medium, repeating the steps for determining the value of the characteristic of the cells and for calculating the number of cells present, calculating the cell growth rate or the variation in the number of anchored cells per unit of time during the period of time from the formula cell variation/t=(N-N.sub.o)/t and comparing the cell growth rate or the variation in the value obtained with a data base of average cell variation per unit time for the same cells in the same medium, wherein the quality of the fluid is below a minimum acceptable if the value is obtained is higher than a predetermined fraction of the corresponding data base value. An in situ method of determining the effectiveness of a biocide on the indigenous cell population of a medium comprises determining the quality of the medium by the above method, and if the quality of the medium is below the minimum acceptable, adding thereto an amount of biocide for a period of time effective to adjust the cell growth rate or the variation in the number of anchored cells per unit time to a predetermined value, and repeating the two previous steps as necessary until the quality of the medium is about at least equal to the minimum acceptable.
REFERENCES:
patent: 2708178 (1955-05-01), Gyorgy
patent: 3684702 (1972-08-01), Hartmann
patent: 4514495 (1985-04-01), Schalkowsky
patent: 4517292 (1985-05-01), Schalkowsky
patent: 4643968 (1987-02-01), Weaver
Microbiology Textbook, Davis et al., 1990, pp. 51-53.
Research and Development Institution, Inc. at Montana State Univ
Rosen Sam
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