Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism
Patent
1987-01-28
1990-06-12
Kepplinger, Esther M.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving viable micro-organism
435 21, C12Q 102, C12Q 142
Patent
active
049332783
ABSTRACT:
A method is disclosed for determining the number of cells in cell culture which comprises lysing the cells and incubating the cell lysate with p-nitrophenyl phosphate for a predetermined period of time at acid pH and at 35.degree.-38.degree. C., and then measuring the color development at 400 to 420 nanometers and comparing with a control. The method is accurate, sensitive, and well-suited for automation, and thereby provides for the rapid sampling of large numbers of cell culture where the cells are grown under very sparse conditions.
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Kepplinger Esther M.
Meyer Scott J.
Monsanto Company
Williams, Jr. James W.
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