Method of determining the nicotine content of tobacco

Details Method of determining the nicotine content of tobacco Method of determining the nicotine content of tobacco

1987-01-13

1988-11-08

Marcus, Michael S.

Chemistry: analytical and immunological testing

Heterocyclic carbon compound

Hetero-n

131297, G01N 3300

Patent

active

047834188

ABSTRACT:
A method of determining on a discrete analyzer the nicotine content of tobacco or a smoking-related product by preparing an aqueous extract of a sample of tobacco or smoking-related product, pre-reacting the extract with an acidic buffered solution of pH not more than 6 for at least two minutes, reacting the pre-reacted extract in the discrete analyzer with aniline and cyanogen bromide, measuring the intensity of yellow coloration generated, and comparing said intensity with that produced by a nicotine standard to determine the nicotine content of said sample.

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W. R. Harvey and A. M. Palmer, Tob. Sci., Mar. 5, 1975, pp. 61-63.
Collins et al., Tob. Abst., vol. 13, No. 1 (13), 1969.
J. A. Allen and W. F. Pickering, Aust. J. Appl. Sci., 12, pp. 42-55, 1961.

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