Data processing: measuring – calibrating – or testing – Measurement system in a specific environment – Biological or biochemical
Reexamination Certificate
2011-07-19
2011-07-19
Skowronek, Karlheinz R (Department: 1631)
Data processing: measuring, calibrating, or testing
Measurement system in a specific environment
Biological or biochemical
C536S025300
Reexamination Certificate
active
07983849
ABSTRACT:
The invention relates to methods of determining the mutational load of a gene bank obtained by random mutagenesis of a gene of interest by preparing a chart linking the mutational load of a gene bank obtained by random mutagenesis of a model gene with the fraction of mutated model genes observed in the bank; performing, in parallel, random mutagenesis of the model gene used for preparing the chart and the gene of interest to obtain the corresponding mutated gene banks; determining the mutational load of the gene bank obtained using the model gene on the basis of the chart plotted; and applying a correction factor to the mutational load of the mutated model gene bank to determine the mutational load in the gene bank of the mutated genes of interest.
REFERENCES:
patent: 2001/0037454 (2001-11-01), Botti et al.
patent: 2003/0152944 (2003-08-01), Hogrefe et al.
Patrick et al. (Protein Engineering, vol. 16, No. 6, p. 451-457, Jun. 2003).
Vu et al. (Nucleic acids research, vol. 28, No. 7, e18, pp. 9, 2000).
Cirino et al. (From: Methods in Molecular Biology, vol. 231: Directed Evolution Library Creation: Methods and Protocols Edited by: F. H. Arnold and G. Georgiou O, Humana Press Inc., Totowa, NJ, p. 3-9, 2003).
Wilson et al.( Current Protocols in Molecular Biology (2000) 8.3.1-8.3.9 Copyright © 2000 by John Wiley & Sons, Inc.).
Chodorge Matthieu
Fourage Laurent
Lefevre Fabrice
Masson Jean-Michel
Hunton and Williams LLP
Proteus S.A.
Skowronek Karlheinz R
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