Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1989-04-26
1992-08-11
Kepplinger, Esther L.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 71, 435 72, 435 79, 435 34, 435 78, 436501, 436827, G01N 33554, G01N 33569, G01N 3353
Patent
active
051378108
ABSTRACT:
A method of determining the gram sign of a bacterial sample is disclosed. In an embodiment of the method, the bacterial sample is first immobilized on a solid support. After immobilization, the bacterial sample is contacted to a peptidoglycan binding ligand (e.g., a ligand which selectively binds to N-acetylglucosamine). The ligand has a detectable label bound thereto. A preferred ligand is wheat germ agglutinin, and a preferred label is fluorescein. After being contacted to the ligand, the bacterial sample is washed with a wash liquid, the wash liquid being provided for a time effective to substantially remove the lectin from gram negative bacteria and ineffective to substantially remove the lectin from gram positive bacteria. The presence or absence of label bound to the bacterial sample is then detected. The presence of label bound to the sample indicates that bacteria in the sample are gram positive bacteria.
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Caldwell Jerra J.
Sizemore Ronald K.
Kepplinger Esther L.
Scheiner Toni R.
The University of North Carolina
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