Method of determining the copy number of a nucleotide sequence

Details Method of determining the copy number of a nucleotide sequence Method of determining the copy number of a nucleotide sequence

2003-07-28

2009-10-20

Horlick, Kenneth R. (Department: 1637)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

Reexamination Certificate

active

07604964

ABSTRACT:
The invention relates to a method of determining of accurately determining the copy number of a nucleotide sequence I in a sample using an amplification technique, such as PCR. In addition, a second nucleotide sequence II is also measured and calibration curves for each are made, from which the relative copy number CN can be determined. According to the present invention, accuracy is improved by performing multiple amplifications in a single well using real time PCR.

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