Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving glucose or galactose
Patent
1987-01-13
1988-10-11
Richman, Barry S.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving glucose or galactose
435 15, 435 18, 435 26, 436131, 436129, 436128, C12Q 154, C12Q 148, C12Q 142, C12Q 132
Patent
active
047771313
ABSTRACT:
A method of determining in a discrete analyzer the total sugars content of a tobacco sample containing sucrose, glucose and fructose, comprises the steps of, converting the sucrose to glucose and fructose, enzymatically converting the glucose and fructose to glucose-6-phosphate in the presence of hexokinase, reacting the glucose-6-phosphate with nicotinamide-adenine dinucleotide in the presence of glucose-6-phosphate dehydrogenase to give reduced nicotinamide-adenine dinucleotide, the amount of reduced nicotinamide-adenine dinucleotide produced being directly proportional to the total sugars content of the sample, measuring the concentration of reduced nicotinamide-adenine dinucleotide in the sample, and generating a signal indicative of the concentration of reduced nicotinamide-adenine dinucleotide and hence of the total sugars content of the sample. Deactivation of enzymes by any cyanogen bromide that may be present is prevented by the addition of dithioerythritol to the reaction mixture.
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"Chemical Abstracts" vol. 91, No. 23, 12/3/79, p. 356, col. 2, No. 189955H; Enzymic determination of glucose, fructose, and sucrose in tobacco. Sekin, Seval. Tob. Sci., 1979, 75-77.
Published European Patent Application No. 8,341A1 (Danninger, et al.), "Process for the Carrying Out of Enzymatic Determinations".
Alsop Susan J.
Long Terence M.
Newman David J.
Imperial Tobacco Limited
McMahon Timothy M.
Richman Barry S.
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