Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism
Patent
1990-07-18
1995-05-09
Wityshyn, Michael G.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving viable micro-organism
435 4, 435 14, 435 29, 435 30, 436 56, 436 57, 436 63, 436173, 424 93, 424 935, C12Q 100, C12Q 102, C12Q 104
Patent
active
054139171
ABSTRACT:
The present invention relates to a method of measuring the contribution of one or more exogenously administered .sup.13 C-labeled substrates to acetyl-CoA. The measurement can be made in a tissue or cell using .sup.13 C NMR without the constraint of metabolic or isotopic steady-state. Furthermore, the method permits the determination even when spectral lines are broad due to B.sub.0 inhomogeneity, thereby opening the way for substrate utilization studies in vivo. The method does not require many of the simplifying assumptions involved in .sup.11 C or .sup.14 C methods, and, since a stable isotope, .sup.13 C, is used a wide variety of compounds with complex labeling patterns may be synthesized and studied.
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Jeffrey F. Mark H.
Malloy Craig R.
Sherry A. Dean
Board of Regents , The University of Texas System
Williams Jane A.
Wityshyn Michael G.
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