Chemistry: analytical and immunological testing – Involving an insoluble carrier for immobilizing immunochemicals
Patent
1990-06-21
1993-03-16
Kepplinger, Esther L.
Chemistry: analytical and immunological testing
Involving an insoluble carrier for immobilizing immunochemicals
435 71, 435 792, 436531, 436532, 436543, 530333, 530334, 530806, 530815, 530826, G01N 33543, G01N 33531, C07K 104
Patent
active
051943921
ABSTRACT:
A method of detecting or determining the sequence of monomers which is a topological equivalent of the epitope which is complementary to a particular paratope of an antibody of interest, comprises the steps of: 1) synthesizing a plurality of catamer preparations; each of the catamer preparations consisting of a plurality of catamers in which the composition at one or more designated positions in each catamer is known, and the composition at the remaining positions is randomly made up from members of a defined set of monomers; and the plurality of catamer preparations comprises preparations in which the composition at the designated positions is systematically varied to contain members from a defined set of monomers; 2) contacting each of the plurality of catamer preparations with the antibody of interest; and 3) detecting or determining the presence or absence of binding between each of the plurality of catamer preparations and the given antibody to indicate a partial sequence of the mimotopes for the paratope of the given antibody.
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Kent, S., Neurath, A. R., Aebersold, R., Parker, K. & Strick, N., "Epitope scanning-Strategies for Determining the Precise Location of Continuous Epitopes Applied to the pre-S Region of HBV", Cold Spring Harbor Symposium, Modern Approaches to Vaccines, Sep. 1985.
Commonwealth Serum Labortories
Kepplinger Esther L.
Scheiner Toni R.
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