Method of determining methotrexate

Details Method of determining methotrexate Method of determining methotrexate

1976-01-20

1977-08-23

Marantz, Sidney

Chemistry: physical processes

Physical processes

Crystallization

23230B, 1951035R, 424 1, G01N 3316, G01N 3106, A61K 4300

Patent

active

040437590

ABSTRACT:
A radioassay system for the determination of methotrexate in biological fluids based on the competitive binding of labeled and unlabeled methotrexate to the enzyme dihydrofolate reductase. Samples of unknown methotrexate level are mixed with I.sup.125 labeled methotrexate. A portion of the total methotrexate present is bound by the addition of enzyme, and the unbound methotrexate is removed with charcoal. The level of bound I.sup.125 labeled methotrexate is measured in a gamma ray counter. To calculate the methotrexate level of the unknown samples, the displacement of bound labeled methotrexate caused by the unknowns is compared to the displacement caused by known methotrexate standards.

REFERENCES:
patent: 3981983 (1976-09-01), Caston
C. E. Myers et al., Proc. Nat. Acad. Sci. USA, 72 (9), 3683-3686, (Sept. 1975).
D. S. Skelly et al., Clinical Chem., 19(2), 146, 147, 148, 150, 157, 158 (1973).
B. Overdijk et al., Clin. Chim. Acta, 59(2), 177-182 (1975).
Chemical Abstracts, 82:92829w (1975).
Chemical Abstracts, 82:106098w (1975).

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