Method of determining metastatic potential of tumor cells

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Glycoprotein – e.g. – mucins – proteoglycans – etc.

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530324, 530350, 530352, 530828, C07K 1400

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055412980

ABSTRACT:
Methods and compositions useful in the detection of human cancer cells and malignant tumors are provided. Certain human autocrine motility factor receptors or proteins (gp78-hAMFR) have been identified which are useful in the detection of human cancer cells and malignant tumors. Methods and assay kits for the detection of human cancer, human cancer cells, and tumors, are provided wherein antibodies or other probes are used which recognize gp78-hAMFR expression. These methods and assay readily distinguish between non-malignant and malignant cancer cells and tumors and can be used to gauge metastatic potential.

REFERENCES:
Nabi, et al., "Identification of B16-F1 Melanoma Autocrine Motility-Like Factor Receptor,"Cancer Research, 50, 409-414, 1990.
Nabi, et al., "Autocrine Motility Factor and its Receptor: Role in Cell Locomotion and Metastasis", Cancer and Metastasis Reviews, 11: 5-20, 1992.
Watanabe, et al., Purification of Human Tumor Cell Autocrine Motility Factor and Molecular Cloning of Its Receptor, The Journal of Biological Chemistry, 266: 13342-13448 (1991).
Watanabe, H., et al., Cancer Research 51, 2699-2705, May 15, 1994, "The Relationship between Motility Factor Receptor Internalization and the Lung Colonization Capacity of Murine Melanoma Cells".

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