Method of determining immunoglobulin levels in mammals

Details Method of determining immunoglobulin levels in mammals Method of determining immunoglobulin levels in mammals

1982-09-30

1985-02-26

Marantz, Sidney

Chemistry: analytical and immunological testing

Rate of reaction determination

436 87, 436517, G01N 3350, G01N 3368, G01N 2182

Patent

active

045018162

ABSTRACT:
A method of determining the presence of abnormal immunoglobulin levels in animal body fluids involves (a) forming a reaction mixture of a body fluid and an effective amount of an appropriate aldehyde; (b) timing the rate of reaction of the aldehyde to gel formation; and (c) comparing the rate of reaction of the reaction mixture to a known rate of reaction of a known mixture of the same aldehyde and body fluid containing a known quantity of an immunoglobulin.

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Tennant B., et al., "Use of the Glutaraldehyde Coagulation Test for Detection of Hypogammaglobulinemia in Neonatal Calves", JAVMA, vol. 174, No. 8, pp. 848-853.
Molla, A., "Immunoglobulin Levels in Calves Fed Colostrum by Stomach Tube", The Veterinary Record, (Oct. 21, 1978).
Sandholm, M., "A Preliminary Report of a Rapid Method for the Demonstration of Abnormal Gammaglobulin Levels in Bovine Whole Blood", Res. Vet. Sci., 1974, 17, (pp. 32-35).
Sandholm, M., et al., "Determination of .gamma.-Globulin in Dog Serum by Glutaraldehyde", J. Small Anim. Practice, (1975), vol. 16, pp. 201-205.
A. Molla, "Estimation of Bovine Colostral Immunoglobulins by Refractometry", The Veterinary Record, (Jul. 12, 1980).
Sandholm, M., "Coagulation of Serum by Glutaraldehyde", Clin. Biochem., vol. 9, No. 1, pp. 39-41, (1976).

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