Method of determining human acid glutathione S-transferase, reag

Details Method of determining human acid glutathione S-transferase, reag Method of determining human acid glutathione S-transferase, reag

1993-12-20

1995-06-27

Nucker, Christine M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 792, 435 794, 435 15, 436 64, 436518, 436548, G01N 3353

Patent

active

054279178

ABSTRACT:
A method of immunologically determining human acid glutathione S-transferase in a human assay sample, which comprises bringing the assay sample into contact with a first antibody bound to an insoluble solid carrier and a labelled second antibody, either the first or second antibody being a polyclonal antibody capable of recognizing human acid glutathione S-transferase or an equivalent fragment of the polyclonal antibody, and the other antibody being a monoclonal antibody capable of specifically recognizing human acid glutathione S-transferase or an equivalent fragment of the monoclonal antibody, a method of diagnosing cancer in a human digestive organ by using the above method, a reagent therefor, a kit therefor and a monoclonal antibody for use therein.

REFERENCES:
patent: 4486530 (1984-12-01), David et al.
patent: 5298393 (1994-03-01), Urushizaki et al.
I. Y. Wang et al., "Multiple Ya Subunits of Glutathione S-Transferase Detected by Monoclonal Antibodies", Articles of Bichemistry and Biophysics, vol. 245, No. 2, Mar. 1986, pp. 543-547.
Shiratori, et al., Cancer Res., 1987, 47(24, Pt. 1), 6806-9, "Immunohistochemical Detection of the Placental Form of Glutathione S-transferase in Dysplastic and Neoplastic Human Uterine Cervix Lesions".

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