Method of determining haptens, use of method and components usef

Details Method of determining haptens, use of method and components usef Method of determining haptens, use of method and components usef

1995-11-20

1997-06-24

Knode, Marian C.

Chemistry: analytical and immunological testing

Involving production or treatment of antibody

Monoclonal antibody

435 71, 435965, 435971, 436518, 436536, 436540, 436822, 5303871, 5303872, 53038825, 5303889, 5303911, 5303913, G01N 3353

Patent

active

056416903

ABSTRACT:
A method is described for determining a hapten which method comprises (i) contacting the hapten with a binding partner of the hapten, whereby the hapten becomes bound to some of the binding partner, (ii) contacting the unbound binding partner with a secondary binding partner therefor, (iii) contacting the binding partner with an antibody which binds the binding partner which has bound thereto the hapten but which does not bind the binding partner which has bound thereto its secondary binding partner; and (iv) determining the amount of antibody bound to the binding partner. Kits for use in the method are also described.

REFERENCES:
patent: 3998943 (1976-12-01), Ullman
patent: 4185084 (1980-01-01), Muchida et al.
patent: 4670383 (1987-06-01), Baier et al.
patent: 4900661 (1990-02-01), Guesdon et al.
patent: 4956303 (1990-09-01), Self
K.M. Gorman et al., Clin Chem, Fol. 37, No. 6, 1991 Antiidiotypic Antibodies as Alternative Labels in Competitive Immunoassays for Thyroxine.
Roitt, "Essential Immunology", Blackwell Scientific Publications, Oxford, England, p. 43, (1977).

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