Method of determining efficiency of ovum collection in bovine

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

Reexamination Certificate

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C435S006110, C435S006120

Reexamination Certificate

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08043817

ABSTRACT:
The novel means by which an efficiency of ovum collection can be easily determined in bovine at gene level is disclosed. The present inventors performed the genomic linkage analysis using bovine populations with high and low efficiency of ovum collection and to identify GRIA1 gene, which encodes an ion channel protein, as a factor deeply related to an efficiency of ovum collection. Bovines having a mutation (e.g. the amino acid substitution of aa306) in GRIA1 produce significantly fewer ova on superovulatory treatment than those not having the mutation. Therefore, the efficiency of ovum collection can be determined based on the existence of a mutation in GRIA1 gene.

REFERENCES:
See NCBI dbSNP submitted SNP (ss) details: ss65441349 obtained from http://www.ncbi.nlm.nih.gov/SNP/snp—ss.cgi?subsnp—id=65441349[Jul. 11, 2011 3:11:19 PM]; two pages.

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