Method of determining DNA sequence preference of a DNA-binding m

Details Method of determining DNA sequence preference of a DNA-binding m Method of determining DNA sequence preference of a DNA-binding m

1995-06-07

1999-02-09

Zitomer, Stepanie W.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435911, 435912, 935 77, 935 78, C12Q 168, C12P 1934

Patent

active

058692410

ABSTRACT:
The present invention defines a DNA:protein-binding assay useful for screening libraries of synthetic or biological compounds for their ability to bind DNA test sequences. The assay is versatile in that any number of test sequences can be tested by placing the test sequence adjacent to a defined protein binding screening sequence. Binding of molecules to these test sequence changes the binding characteristics of the protein molecule to its cognate binding sequence. When such a molecule binds the test sequence the equilibrium of the DNA:protein complexes is disturbed, generating changes in the concentration of free DNA probe. Numerous exemplary target test sequences (SEQ ID NO:1 to SEQ ID NO:600) are set forth. The assay of the present invention is also useful to characterize the preferred binding sequences of any selected DNA-binding molecule.

REFERENCES:
patent: 4257774 (1981-03-01), Richardson et al.
patent: 4270924 (1981-06-01), Crooke et al.
patent: 5071773 (1991-12-01), Evans et al.
patent: 5096815 (1992-03-01), Ladner et al.
Weideli et al. Eur. J. Biochem. 104:5-11 (1980).
Cordonnier et al. Nucleic Acids Research 15(2): 477-490 (1987).
Gardner et al. J. Biol. Chem. 254(12): 5238-5242 (1979).
Norby et al. Nucleic Acid Research 20(23): 6317-6321 (1992).
Diffley et al. Molecular and Cellular Biol. 6(5): 1363-1373 (1986).
Davison, A.J., and Scott, J.E., "The Complete DNA Sequence of Varicella-Zoster Virus," J. Gen. Virol. 67:1759-1816 (1986).
Hanvey, J. C., et al., "Site-Specific Inhibition of EcoR1 Restriction/Modification Enzymes by a DNA Triple Helix," Nuc. Acids Res. 18(1):157-161 (1990).
Hobson, K., et al., "Use of DNA-Protein Interaction to Isolate Specific DNA Sequences," Anal. Biochem. 193:220-224 (1991).
McGeoch, D.J., et al., "The Complete DNA Sequence of the Long Unique Region in the Genome of Herpes Simplex Virus Type 1," J. Gen. Virol. 69:1531-1574 (1989).
Ray, R., et al., "Mithramycin Blocks Protein Binding and Function of the SV40 Early Promoter," J. Clin. Invest. 83:2003-2007 (1989).
Snyder, R. C., et al., "Mithramycin Blocks Transcriptional Initiation of the c-myc P1 and P2 Promoters," Biochemistry 30:4290-4297 (1991).
Van Dyke, M.W., and Dervan, P.B., "Chromomycin, Mithramycin, and Olivomycin Binding Sites on Heterogeneous Deoxyribonucleic Acid--Footprinting with (Methidiumpropyl-EDTA)ironIII), " Biochemistry 22:2273-2377 (1983).

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