Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Reexamination Certificate
2006-02-28
2006-02-28
Fredman, Jeffrey (Department: 1637)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
C435S091200, C435S091210, C435S091500, C435S006120
Reexamination Certificate
active
07005278
ABSTRACT:
The present invention relates to prognostic methods which are useful in medicine, particularly cancer chemotherapy. The object of the invention to provide a method for assessing Dihydropyrimidine dehydrogenase (DPD) expression levels in tissues and prognosticate the probable resistance of a patient's tumor to treatment with 5-FU based therapies by examination of the amount of DPD mRNA in a patient's tumor cells and comparing it to a predetermined threshold expression level. More specifically, the invention provides to oligonucleotide primer pairs DPD3A and DPD3B and methods comprising their use for detecting levels of Dihydropyrimidine dehydrogenase (DPD) mRNA.
REFERENCES:
patent: 4830969 (1989-05-01), Holmes
patent: 4843155 (1989-06-01), Chomczynski
patent: 5128247 (1992-07-01), Koller
patent: 5284940 (1994-02-01), Lin et al.
patent: 5346994 (1994-09-01), Chomczynski
patent: 5502166 (1996-03-01), Mishina
patent: 5620852 (1997-04-01), Lin et al.
patent: 5637687 (1997-06-01), Wiggins
patent: 5643767 (1997-07-01), Fischetti et al.
patent: 5654179 (1997-08-01), Lin
patent: 5672696 (1997-09-01), Wang et al.
patent: 5707802 (1998-01-01), Sandhu et al.
patent: 5728822 (1998-03-01), Macfarlane
patent: 5777099 (1998-07-01), Mehra
patent: 5856454 (1999-01-01), Gonzalez et al.
patent: 5945515 (1999-08-01), Chomczynski
patent: 5989857 (1999-11-01), Mundschenk
patent: 6010700 (2000-01-01), Richt
patent: 6043354 (2000-03-01), Hillebrand et al.
patent: 198 37 391 (1999-02-01), None
patent: WO 95/28489 (1995-10-01), None
patent: WO 96/08568 (1996-03-01), None
patent: WO 97/05248 (1997-02-01), None
patent: WO 97/35034 (1997-09-01), None
patent: WO 98/41648 (1998-09-01), None
patent: WO 02/44423 (2002-06-01), None
Buck et al, “Design strategies and performance of custom DNA sequencing primers”, Biotechniques (1999) 27(3):528-536.
Peng et al, “Multiple PCR analyses on trace amounts of DNA extracted from fresh and paraffin wax embedded tissues after random hexamer primer PCR amplification”, J. Clin. Pathol. 47(7):605-8.
WIllhauck et al, “Internal control for quality assurance of Diagnostic RT-PCR”, Biotechniques (Oct. 1998) 25(4):656-659.
Selvey et al, B-Actin an unsuitable internal control for RT-PCT (Mol. Cell. Probes (2002) 15:307-311).
G. Stanta, et al.,RNA Quantitative Analysis from Fixed and Paraffin-Embedded Tissues,Methods in Molecular Biology (1998) vol. 86, pp. 113-119.
G. Stanta, et al.,RNA Extraction from Formalin-Fixed and Paraffin-Embedded Tissues,Methods in Molecular Biology (1998) vol. 86, pp. 23-26.
Aschele, C., et al., “Thymidylate Synthase Protein Expression in Primary Colorectal Cancer Compared with the Corresponding Distant Metastases and Relationship with the Clinical Response to 5-Fluorouracil,” Clinical Cancer Research, 6:4797-4802 (2000).
Chen, Z., et al., “Correlation of Cisplatin Sensitivity with Differential Alteration of EGFR Expression in Head and Neck Cancer Cells,” Anticancer Research 20:899-902 (2000).
Gorlick, R., et al., “Higher Levels of Thymidylate Synthase Gene Expression Are Observed in Pulmonary as Compared With Hepatic Metastases of Colorectal Adenocarcinoma,” Journal of Clinical Oncology, 16(4):1465-1469 (1998).
Kornmann M. et al., “Thymidylate synthase is a predictor for response and resistance in hepatic artery infusion chemotherapy,” Cancer Letters, 118:29-35 (1997).
Lenz, H.-J., et al., “p53 Point Mutations and Thymidylate Synthase Messenger RNA Levels in Disseminated Colorectal Cancer: An Analysis of Response and Survival,” Clinical Cancer Research, 4:1243-1250 (1998).
Neskovic-Konstantinovic, Z., et al., “Expression of Epidermal Growth Factor Receptor in Breast Cancer, from Early Stages to Advanced Disease,” J. Exp. Clin. Cancer Res., 18(3):347-355 (1999).
Newby J.C., et al., “Expression of Epidermal Growth Factor Receptor and c-erbB2 during the Development of Tamoxifen Resistance in Human Breast Cancer,” Clinical Cancer Research, 3:1643-1651 (1997).
Nicholson, R.I., et al., “Relationship Between EGF-R, c-erbB-2 Protein Expression and Ki67 Immunostaining in Breast Cancer and Hormone Sensitivity,” Eur. J. Cancer, 29A(7):1018-1023 (1993).
Wong, NACS, et al., “Nuclear thymidylate synthase expression, p53 expression and 5FU response in colorectal carcinoma,” British Journal of Cancer, 85(12):1937-1943 (2001).
Yamada, H., et al., “Thymidylate Synthase Gene Expression in Primary Colorectal Cancer and Metastatic Sites,” Clinical Colorectal Cancer, 1(3):169-173 (2001); discussion p. 174.
Ardalan, B., et al., “Thymidylate Synthase Gene Expression in Normal and Malignant Colorectal Tissues: Relation to in vivo Response and Survival,” Proceedings of the American Association for Cancer Research, vol. 37, Abstract No. 1376 (Mar. 1996).
Ausubel, F.M., ed.,Current Protocols In Molecular Biology,John Wiley & Sons, Inc., vol. 1.; Strauss, W. M., “Preparation of Genomic DNA from Mammalian Tissue,” Unit 2.2.1-2.2.3 (1998).
Ausubel, F.M., ed.,Current Protocols In Molecular Biology,John Wiley & Sons, Inc., vol. 1.; Richards, E., et al., “Preparation of Genomic DNA from Plant Tissue,” Unit 2.3, pp. 2.3.1-2.3.7 (1994).
Ausubel, F.M., ed.,Current Protocols In Molecular Biology,John Wiley & Sons, Inc., vol. 1.;Wilson, K., “Preparation of Genomic DNA from Bacteria,” Unit 2.4, pp. 2.4.1-2.4.5 (1994).
Banerjee, S.K., et al, “Microwave-Based DNA Extraction from Paraffin-Embedded Tissue for PCR Amplification,” BioTechniques, vol. 18, No. 5, pp. 768, 770, 772, 773 (1995).
Benhatter, J., et al., “p53 Mutations as a Possible Predictor of Response to Chemotherapy in Metastatic Colorectal Carcinomas,” Int. J. Cancer (Pred. Oncol.), vol. 69, pp. 190-192 (1996).
Bresters, D., et al., “Detection of Hepatitis C Viral RNA Sequences in Fresh and Paraffin-Embedded Liver Biopsy Specimens of Non-A, Non-B Hepatitis Patients,” J. Hepat., vol. 15, pp. 391-395 (1992).
Bresters, D., et al., “The Duration of Fixation Influences the Yield of HCV cDNA-PCR Products from Formalin-fixed, Paraffin-Embedded Liver Tissue,” J. Virol. Method, vol. 48, pp. 267-272 (1994).
Chirgwin, J., M., et al., “Isolation of Biologically Active Ribonucleic Acid from Sources Enriched in Ribonuclease,” Biochemistry,vol. 18, No. 24, pp. 5294-5299 (1979).
Chomczynski, P., et al., “Single-Step Method of RNA Isolation by Acid Guanidinium Thiocyanate-Phenol-Chloroform Extraction,” Analytical Biochemistry, vol. 162, pp. 156-159 (1987).
Chomczynski, P., “A Reagent for the Single-Step Simultaneous Isolation of RNA, DNA and Proteins from Cell and Tissue Samples,” BioTechniques, vol. 15, No. 3, pp. 532-534, 536-537 (1993).
Coombs, N.J., et al., “Optimisation of DNA and RNA Extraction from Archival Formalin-Fixed Tissue,” Nucl. Acids Research, vol. 27, No. 16, pp. i-iii (1999).
Dakhama, A., et al., “Amplification of Human β-Actin Gene by the Reverse Transcriptase-Polymerase Chain Reaction: Implications for Assessment of RNA from Formalin-Fixed, Paraffin-Embedded Material,” J. Histochem. and Cytochem., vol. 44, No. 10, pp. 1205-1207 (1996).
de Andrés, B., et al., “Improved Method for mRNA Extraction from Paraffin-Embedded Tissues,” BioTechniques, vol. 18, No. 1, pp. 42-43 (1995).
Eads, C. A., et al., “CpG Island Hypermethylation in Human Colorectal Tumors is Not Associated with DNA Methyltransferase Overexpression,” Cancer Research, vol. 59, pp. 2302-2306 (1999).
Edamoto, Y., et al., “Hepatitis C and B Virus Infections in Hepatocellular Carcinoma,” Cancer, vol. 77, No. 9, pp. 1787-1791 (1996).
Farrugia, D., et al., “A Pharmacodynamic(PD)study of the thymidylate, synthase(TS)Inhibitor Tomudex™ in advanced colorectal cancer(CRC),” Proceedings of the American Association for Cancer Research, E
LandOfFree
Method of determining dihydropyrimidine dehydrogenase gene... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method of determining dihydropyrimidine dehydrogenase gene..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method of determining dihydropyrimidine dehydrogenase gene... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3646919