Chemistry: molecular biology and microbiology – Plant cell or cell line – per se ; composition thereof;... – Culture – maintenance – or preservation techniques – per se
Patent
1999-02-19
2000-11-21
Lankford, Jr., Leon B.
Chemistry: molecular biology and microbiology
Plant cell or cell line, per se ; composition thereof;...
Culture, maintenance, or preservation techniques, per se
435 4, 435430, 4354301, C12N 504
Patent
active
061501670
ABSTRACT:
The invention relates to the knowledge of sugar alcohols as indicators of biochemical maturity of pine zygotic and somatic embryos. It has been discovered that loblolly pine zygotic embryos have a progression of different sugar alcohols as they approach biochemical maturity. Pinitol is the first sugar alcohol to appear in abundance. This is followed by D-chiro-inositol, and finally by fagopyritol B1. As a later appearing alcohol becomes more abundant in the embryos, the earlier ones decrease in amount present. Analysis of the embryo sugar alcohols can be helpful in determining when to harvest cones from seed orchards. Further it can be used as a guideline for formulation of more effective culture media in the propagation of somatic embryos by tissue culture. There appears to be a requirement for fagopyritol B1 if good germination of somatic embryos is to be achieved.
REFERENCES:
patent: 5036007 (1991-07-01), Gupta et al.
patent: 5413930 (1995-05-01), Becwar et al.
patent: 5464769 (1995-11-01), Attree et al.
patent: 5491090 (1996-02-01), Handley et al.
patent: 5506136 (1996-04-01), Becwar et al.
patent: 5677185 (1997-10-01), Handley et al.
patent: 5731191 (1998-03-01), Rutter et al.
patent: 5731203 (1998-03-01), Handley et al.
patent: 5731204 (1998-03-01), Rutter et al.
Baron, Frank J. Metabolic patterns in dormant and germinating seeds of sugar pine (Pinus lambertiana, Dougl.). In Advancing Frontiers of Plant Sciences, Lo-kesh Chandra, ed., 24:47-63 (1970).
Bernabe, M., R. Fenwick, J. Frias, J. Jimenez-Barbero, K. Price, S. Valverde, and C. Vidal-Valverde. Determination, by NMR spectroscopy, of the structure of ciceritol, a pseudotrisaccharide isolated from lentils. Journal of Agricultural and Food Chemistry 41: 870-872 (1993).
Chien, C.-T., T.-P. Lin, C.-G. Jou, and G.-R. Her. Occurrence of a novel galactopinitol and its changes with other non-reducing sugars during development of Leucaena leucocephala seeds. Plant Cell Physiology 37: 539-544 (1996).
Gorecki, R. J., A. I. Piotrowicz-Cieslak, L. B. Lahuta, and R. L. Obendorf. Soluble carbohydrates in desiccation tolerance of yellow lupin seeds during maturation and germination. Seed Science Research 7: 107-115 (1997).
Gupta, P. K. and D. J. Durzan. Biotechnology of somatic polyembryogenesis in plantlet regeneration in loblolly pine. Bio/Technology 5: 147-151 (1987).
Handley, L. W., L. W. Pharr, and R. F. McFeeters. Relationship between galactinol synthase activity and sugar compositions of leaves and seeds of several crop species. Journal of the American Society of Horticultural Science 108(4) 600-605 (1983).
Horbowicz, M., P. Brenac, and R. L. Obendorf. Fagopyritol B1, O-.alpha.-D-galactopyranosyl-(1.fwdarw.2)-D-chiro-inositol, a galactosyl cyclitol in maturing buck-wheat seeds associated with desiccation tolerance. Planta 205: 1-11 (1998).
Horbowicz, M. and R. L. Obendorf. Seed desiccation tolerance and stability: Dependence on flatulence producing oligosaccharides and cyclitols--review and survey. Seed Science Research 4: 358-405 (1994).
Horbowicz, M., R. L. Obendorf, B. D. McKersie, and D. R. Viands. Soluble saccharides and cyclitols in alfalfa (Medicago sativa L.) somatic embryos, leaflets, and mature seeds. Plant Science 109: 191-198 (1995).
Kuo, T. M. Isolation and Identification of galactinol from castor oilseed meal. Journal of the American Oil Chemists Society 69: 569-574 (1992).
Kuo, T. M., C. A. Lowell, and T. C. Nelsen. Occurrence of pinitol in developing soybean seed tissues. Phytochemistry 45: 29-35 (1997).
Murphy. J. B. and M. F. Hammer. Respiration and soluble sugar metabolism in sugar pine embryos. Physiologia Plantarum 74: 95-100 (1988).
Obendorf, R. L. Oligosaccharides and galactosyl cyclitols in seed desiccation tolerance. Seed Science Research 7: 63-74 (1997).
Obendorf, R. L., M. Horbowicz, A. M. Dickerman, P. Brenac, and M. E. Smith. Soluble oligosaccharides and galactosyl cyclitols in maturing soybean seeds In planta and In vitro, Crop Science 38: 78-84 (1998).
peterbauer, T. and A. Richter. Galactosylononitol and stachyose synthase in seeds of adzuki bean. Plant Physiology 117: 165-172 (1998).
Phillipy, Brian Q. Identification of inositol 1,3,4-triphosphate 5 kinase and inositol 1,3,4,5-tetrakisphosphate 6-kinase in immature soybean seeds. Plant physiology 116: 291-297 (1998).
Teasdale, R. D., P. A. Dawson, and H. W. Woolhouse. Mineral nutrient requirements of loblolly pine. (Pinus taeda cell suspension culture). Plant physiology 82: 942-945 (1986).
Carpenter Carolyn V.
Koester Martha K.
Lankford , Jr. Leon B.
Weyerhaeuser Company
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