Method of determining amino acid sequence of protein or peptide

Details Method of determining amino acid sequence of protein or peptide Method of determining amino acid sequence of protein or peptide

1992-08-18

1996-05-28

Redding, David A.

Chemistry: analytical and immunological testing

Peptide, protein or amino acid

436 89, 436173, 530334, 530342, 530343, 530344, 530402, 530412, G01N 3300

Patent

active

055210970

ABSTRACT:
A protein or peptide is treated by a vapor containing an organic acid represented by the following general formula CF.sub.3 --(CF.sub.2)n--COOH (n is zero or more integer). The resulting reaction mixture is processed by a mass spectrometer to obtain a mass spectrum to measure a mass of respective chemical species contained in the reaction mixture. Alternatively, the reaction mixture is processed by an amino acid analyzer to determine an amino acid sequence of the protein or peptide from the carboxy-terminal. According to another method, the protein or peptide is treated by an anhydride of the organic acid represented by the following general formula CF.sub.3 --(CF.sub.2)n--COOH (n is zero or more integer). The resulting reaction mixture is processed by a mass spectrometer to obtain a mass spectrum to measure a mass of respective chemical species contained in the reaction mixture to determine an amino acid sequence of the protein or peptide from the carboxy-terminal.

REFERENCES:
patent: 4701419 (1987-10-01), Morris
patent: 4820648 (1989-04-01), Caprioli et al.
patent: 5104973 (1992-04-01), Kondo et al.
patent: 5185266 (1993-02-01), Boyd et al.
Orlovska et al., "Use of trifluoroacetic acid and its anhydride for studying the N-terminal sequence of amino acids in proteins", Chemical Abstracts, vol. 70, No. 1 (1969), p. 68.
Hawke et al., "Microsequence Analysis of Peptides and Proteins: Trimethyl-silylisothiocyanate as a Reagent for COOH-Terminal Sequence Analysis", Analytical Biochemistry, vol. 166, No. 2, Nov. 1, 1987, pp. 298-307.
Tsugita et al., "Reaction of Pentafluoropropionic Anhydride Vapor on Polypeptide as Revealed by Mass Spectrometry. A Carboxypeptidase Mimetic Degradation", Chemistry Letters, vol. 2, 1992, pp. 235-238.
Tsugita et al., "Exopeptidase Digestion in Combination with Field Desorption Mass Spectrometry for Amino Acid Sequence Determination", FEBS Letters, vol. 137, No. 1, Jan. 1982, pp. 19-24.

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