Method of determining a viable count using a hydrophobic membran

Details Method of determining a viable count using a hydrophobic membran Method of determining a viable count using a hydrophobic membran

1995-05-18

1998-06-16

Tsang, Cecilia J.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving luciferase

435 4, 435 29, 435 34, 435259, 4352911, 436172, 436177, 422 52, 422 826, 210650, 210651, C12Q 166, G01N 2176, B01D 6100

Patent

active

057668680

ABSTRACT:
An improved method of determining a viable microbial cell count in a sample solution with an enhanced detection sensitivity is provided, comprising filtering the sample solution through a filtration membrane having hydrophobic properties to entrap microbes within hydrophobic barriers; applying thereto a fine spray of ATP releasing reagent to extract a luminescent ingredient from the microbes; applying thereto another fine spray of liquid luminescence-inducing reagent to allow the released luminescent ingredient to emit luminescence; and measuring the level of the luminescence, using a competent means for measuring the luminescence level. The microbes and the two types of reagents are locally concentrated due to the hydrophobic properties of the membrane and thus the sensitivity is greatly enhanced, permitting instant counting in the case of large microbes, and decreased culture time for smaller bacterial microbes.

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Tsai et al, Proc. Soc. Exp. Biol. Med., vol. 183, No. 1, pp. 74-80, Oct. 1986.
Seto et al, Chemical Abstracts, vol. 118, p. 491, Ref. #208986 p. 1993.

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