Method of determination of fluorescent substance and method of a

Details Method of determination of fluorescent substance and method of a Method of determination of fluorescent substance and method of a

1997-04-24

1998-08-11

Snay, Jeffrey

Chemistry: analytical and immunological testing

Composition for standardization, calibration, simulation,...

436172, 435 4, 2504591, G01N 2164

Patent

active

057926620

ABSTRACT:
A method for determining a fluorescent substance in a broader concentration range with higher measurement accuracy is provided which is applicable to assay of enzyme activity in enzyme-label immunoassay, enzyme-label DNA hybridization, etc. A method for assaying enzyme activity is also provided which employs the above fluorescence measurement. In the method, a specimen containing an objective fluorescent substance to be determined and a reference substance is irradiated with excitation light capable of exciting both the objective fluorescent substance and the reference fluorescent substance; measuring a first fluorescence intensity at a first wavelength where fluorescence is emitted mainly by the objective fluorescent substance; measuring a second fluorescence intensity at a second wavelength where fluorescence is emitted mainly by the reference fluorescent substance; and calculating the concentration of the objective fluorescent substance from the equation:

REFERENCES:
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patent: 5460943 (1995-10-01), Hayashi et al.
Mickley, H.S. et al. "Applied Mathematics in Chemical Engineering" Second Edition, McGraw Hill Book Company, New York, pp. 8-15 (1957).

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