Method of determination of calcium

Details Method of determination of calcium Method of determination of calcium

1995-04-20

1997-04-08

Kight, John

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving transferase

435 4, 435 13, 435 15, 435110, 435183, 436 63, 436 74, 436 69, 530300, 530328, C12Q 152, C12Q 100, C12Q 148, G01N 3348

Patent

active

056186843

ABSTRACT:
Calcium in a sample is brought into contact with a transglutaminase capable of being activated with calcium as an activating factor and the transglutaminase activity, which varies depending upon the calcium amount in the sample, is measured to thereby determine the calcium amount in the sample. By the method of the invention, accurate determination of calcium in various samples such as body fluids is possible without removal of proteins from them.

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patent: 5204240 (1993-04-01), Stuber
patent: 5204447 (1993-04-01), Bishop et al.
Lipmann et al., A Specific Micromethod for the Determination of Acyl Phosphates, J. Biol. Chem., vol. 159, No. 21, 1954.
23rd General Meeting of the Association of Japanese Journal of Clinical Laboratory Automation, vol. 16, pp. 222-223, 1991.
Folk et al, Molecular and Catalytic Properties of Transglutaminases, Adv. Enzymol., vol. 38, pp. 109-191, 1973.
Hand et al, Activation of Transglutaminase at Calcium Levels Consistent with a Role for this Enzyme as a Calcium Receptor Protein, Biological Abstracts, vol. 81, No. 11, 1986.
Muszbek et al, Kinetic Determination of Blood Coagulation Factor XIII in Plazma, Clinical Chemistry, vol. 31, No. 1, Jan. 1985, Winston US pp. 35-40.
Klein et al, Jour. of Bacteriology, Apr. 1992, pp. 2599-2605.
A Referee Method for the Determination of Total Calcium in Serum, Clinical Chemistry, vol. 19, No. 10, pp. 1208-1213, 1973.

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