METHOD OF DETECTION IN VITRO OF A TARGET SUBSTANCE IN A...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

Reexamination Certificate

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C435S069100, C435S091200

Reexamination Certificate

active

06911307

ABSTRACT:
The present invention relates to a method of detection in vitro of a target substance in a sample. The sample can comprise, among other things, a nucleic sequence or more generally any type of substance. In particular, the present invention provides a method of detection comprising (i) specificly labeling a substance with a reporter gene and any sequences necessary for the in vitro expression of the reporter gene; (ii) in vitro transcription and translation of the reporter gene; and (iii) in vitro detection of a reporter protein coded by the reporter gene.

REFERENCES:
patent: 4800159 (1989-01-01), Mullis et al.
patent: 5602301 (1997-02-01), Field
patent: 5952201 (1999-09-01), Landegren et al.
patent: 5972609 (1999-10-01), Tinsley et al.
patent: 518557 (1992-12-01), None
patent: 2276621 (1994-10-01), None
patent: WO 92/07949 (1992-05-01), None
patent: WO 94/03630 (1994-02-01), None
patent: WO 94/05812 (1994-03-01), None
patent: WO 94/06928 (1994-03-01), None
patent: WO 96/01327 (1996-01-01), None
patent: WO 96/08580 (1996-03-01), None
patent: WO 97/19193 (1997-05-01), None
patent: WO 98/11249 (1998-03-01), None
patent: WO 98/27225 (1998-06-01), None
Watson J.D. et al Recombinant DNA 2nd edition, 1992, WH Freeman & Company p. 155.
Gibco BRRL products & reference Guide pp. 15-6, 19-4, 19-5 1997.
Amersham Catalogue pp. 116 1998.
Ohuchi et al Nucleic Acids Research vol. 26 No. 19 pp. 4339-4346 1998.
Sarkar et al Biotechniques vol. 8 No. 4 pp. 404-407 1990.
Cassata, G. et al., “Rapid Expression Screening of Caenorhabditis Elegans Homeobox Open Reading Frames Using a Two-Step Polymerase Chain Reaction Promoter-gfpReporter Construction Technique”,Gene, vol. 212; pp. 127-135 (1998).
Hanes, J. et al., “In Vitro Selection and Evolution of Functional Proteins by Using Ribosome Display”,Proc. Natl. Acad. Sci., vol. 94, pp. 4937-4942 (1997).
Resto et al., “Amplification of Protein Expression in a Cell Free System”,Nucleic Acids Research, vol. 20, No. 22, pp. 5979-5983 (1992).
Sun, Y. et al., “Comparison of Peptide Enzyme-Linked Immunosorbent Assay and Radioimmunoprecipitation Assay with In Vitro-Translated Proteins for Detection of Serum Antibodies to Human Papillomavirus Type 16 E6 and E7 Proteins”,Journal of Clinical Microbiology, vol. 32. No.9, pp. 2216-2220 (1994).

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

METHOD OF DETECTION IN VITRO OF A TARGET SUBSTANCE IN A... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with METHOD OF DETECTION IN VITRO OF A TARGET SUBSTANCE IN A..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and METHOD OF DETECTION IN VITRO OF A TARGET SUBSTANCE IN A... will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-3464120

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.