Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1994-01-14
1997-10-14
Zitomer, Stephanie W.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 912, 935 77, 935 78, C12P 1934, C12Q 168
Patent
active
056771258
ABSTRACT:
The present invention provides a method of detecting and diagnosing pre-invasive breast cancer by identifying differentially expressed genes in early, pre-invasive breast cancer tissue. Differentially expressed genes can be used as genetic markers to indicate the presence of pre-invasive cancerous tissues. Microscopically-directed tissue sampling techniques combined with differential display or differential screening of cDNA libraries are used to determine differential expression of genes in the early stages of breast cancer. Differential expression of genes in preinvasive breast cancer tissue is confirmed by RT-PCR, nuclease protection assays and in-situ hybridization of ductal carcinoma in situ tissue RNA and control tissue RNA.
REFERENCES:
Holt JT, RA Jensen, and DL Page. Histopatholoy: Old Principles and new methods. 1994. Cancer Surveys. vol. 18: A New Look at Breast Cancer.
Liang P, and AB Pardee. 1992. Differential display of eukaryotic mRNA by means of the polymerase chain reaction. Science 257:967-971.
Sanchez LM, F Vizoso, I Diez-Itza, C Lopez-Otin. 1992. Cancer Res 32:95-100.
Dupont WD, and DL Page. 1985. Risk factors for beast cancer in women with proliferative breast disease. N.Engl.J.Med. 312:146-151.
Davis et al., Basic Methods in Molecular Biology, Elsevier, NY 1986 pp. 276-284.
Kang et al. Science 229:974-978 (1985).
Marx, Science 244:654-655 (12 May 1989).
Slamon et al., Science 244:256-262 (12 May 1989).
Singleton et al., Dictionary Microbiology and Molecular Biology (1987) pp. 783, 889, 890.
Holt Jeffrey T.
Jensen Roy A.
Obermiller Patrice S.
Page David L.
Robinson-Benion Cheryl L.
Patterson Mark J.
Taylor, Jr. Arles A.
Vanderbilt University
Zitomer Stephanie W.
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