Method of detecting β-D-ribofuranosidase activity

Details Method of detecting β-D-ribofuranosidase activity Method of detecting β-D-ribofuranosidase activity

2008-06-10

2008-06-10

Gitomer, Ralph (Department: 1657)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving viable micro-organism

C435S018000

Reexamination Certificate

active

07384763

ABSTRACT:
This invention provides new methods for detecting enzyme activity using enzyme substrates comprising an enzyme cleavable portion and a chromogenic portion. The enzyme cleavable portion is a β-D-ribofuranosyl group and the chromogenic portion forms a detectable indicator following enzyme cleavage. In one aspect of this invention the chromogenic β-D-ribofuranoside substrate forms a colored substantially non-diffusable indicator. A second aspect of this invention provides novel chromogenic β-D-ribofuranosides.

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