Method of detecting transmissible spongiform encephalopathies

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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424 2, 424 5, 435 792, 435 793, 436149, 436811, G01N 3353, G01N 100, A61K 4904

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059981490

ABSTRACT:
This invention provides an improved assays for the detection of transmissible spongiform encephalopathies (TSEs) in humans and non-human mammals. The methods involve detecting the presence or absence of 14-3-3 proteins in cerebrospinal fluid from the tested organism. Elevated levels of 14-3-3 are indicative of transmissible spongiform encephalopathies, in particular Creutzfeldt-Jacob disease in humans or mad cow disease in bovines).

REFERENCES:
patent: 4892814 (1990-01-01), Harrington et al.
patent: 5270165 (1993-12-01), Van Nostrand et al.
Ichimura et al "Widespread Distributuion of the 14-3-3 Protein in Vertibrate Brains and Bovine Tissues: Correlation with the Distribution of Calcium-Dependent Protein Kinases" J. Neurchem 56:1449-1541 (1991).

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