Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving virus or bacteriophage
Patent
1986-06-12
1992-02-04
Saunders, David A.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving virus or bacteriophage
435 6, 435 71, 435 75, 435 29, 436501, 436518, C12Q 168, C12Q 170, G01N 33543
Patent
active
050859820
ABSTRACT:
The invented method utilizes a signal amplification system comprising living cells which are specifically provided with the ability to survive, reproduce and be detected in the event that a target molecule is present. The method comprises first the step of binding a target molecule to a substratum. In the second step a phagemid is prepared which is capable of transfecting a cell enabling such transfected cell to produce a signal, such as color or light. The phagemid is then provided with a means for binding to a probe, thus forming a phagemid complex.
The probe may also be provided with a means of binding to the phagemid complex. This modified probe is then hybridized with the target molecules bound to the substratum. Thereafter, the phagemid complex is hybridized with the modified probe and specific binding occurs between the phagemid complex and the modified probe thus forming a substrutum-target-probe phagemid complex. As a next step, lysogenic bacterial cells which are infectable by the particular phagemid being used are added to the system in an environment which favors transfection of the phagemid nucleic acid into the bacteria. The growth medium is constituted so that only transfected bacteria survive and are capable of reproducing. The phagemid also enable the bacteria to produce a signal such light or color. Only the transfected bacterial cells containing the plasmid gene for producing the signal will be detectable.
REFERENCES:
patent: 4104126 (1978-08-01), Young
patent: 4321365 (1982-03-01), Wu et al.
patent: 4746604 (1988-05-01), Mowshowitz
Hamada et al., Gene, 24, 245-253, 1983.
City of Hope
Irons Edward S.
Saunders David A.
LandOfFree
Method of detecting specific substances by selective growth of l does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method of detecting specific substances by selective growth of l, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method of detecting specific substances by selective growth of l will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-347543