Method of detecting red cell antigen-antibody reactions

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

Reexamination Certificate

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C435S002000, C435S287200, C436S506000, C436S507000, C436S513000, C436S518000, C436S520000, C436S538000, C436S539000, C436S017000, C436S018000, C436S165000, C436S166000, C436S175000, C436S177000, C436S178000, C436S179000

Reexamination Certificate

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07919266

ABSTRACT:
A process for the detection of antibodies in a test sample by preparing a suspension of erythrocytes with a test serum or plasma by mixing a test serum or plasma with erythrocytes; incubating the suspension of erythrocytes at a temperature of from 37° C. to 45° C. to bind any antibodies in the test serum or plasma to the surface of said erythrocytes; combining the suspension of erythrocytes with an amount of a solution of a macromolecule which is effective to agglutinate the erythrocytes; packing the resultant red cell agglutinates by centrifuging the suspension of erythrocytes; and, determining the presence of anti-erythrocyte antibodies by observing if antibody-dependent erythrocyte agglutination has occurred.

REFERENCES:
patent: 4436825 (1984-03-01), Lalezari
patent: 2007/0298446 (2007-12-01), Malyska et al.
British Journal of Experimental Pathology, Vo. 26, pp. 255-266 (1945).

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