Method of detecting pseudorabies virus specific serum antibody b

Details Method of detecting pseudorabies virus specific serum antibody b Method of detecting pseudorabies virus specific serum antibody b

1989-01-23

1992-06-16

Kepplinger, Esther L.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving virus or bacteriophage

435 792, 4352351, 424 89, G01N 33569

Patent

active

051224470

ABSTRACT:
A method of testing serum from swine vaccinated against pseudorabies virus with viral envelope-based subunit vaccines to determine the presence of antibodies to infecting pseudorabies virus in wihch an immunoassay is performed on the swine serum using a pseudorabies virus antigen preparation comprising nucleocapsid proteins of the pseudorabies virus. The universal diagnostic antigen is one or more nucleocapsid proteins having relative molecular weights of approximately 23 k, 34 k, 41 k, 63 k and 140 k.

REFERENCES:
patent: 4810634 (1989-03-01), Post et al.
Platt, Vet. Microb. 9:35-51 (1984), The Evaluation of a Lectin-Agarose Based Subunit Vaccine and Complementary Diagnostic Antigen for Aujeszky's Disease (Pseudorabies) in the Pig.
Stevely, J. of Virol. 16:944-950 (1975), Virus-Induced Proteins in Pseudorabies-Infected Cells.
McGinley and Platt, Am. J. of Vet. Res. 50:1290-1293, Antibody Response of Pseudorabies Virus Subunit-Vaccinated Pigs to Viral Nucleocapsid Proteins Following Low-Dose Virus Challenge of Immunity, (Aug. 1989).
Van Oirschot et al., "Differentiation of Serum Antibodies from Pigs Vaccinated or Infected with Aujeszky's Disease Virus by a Competitive Enzyme Immunoassay", J. Gen. Virol. 67: 1179-1182 (1986).
Herbrink et al., "The Antigen Spot Test (AST): A Highly Sensitive Assay for Detection of Antibodies" J. Immunol. Methods 48: 293-298 (1982).

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