Method of detecting proteolytically modified antithrombin

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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435 13, 435962, 435967, 435 794, G01N 3353

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active

052485960

ABSTRACT:
Indirect method of detecting elastase-modified or cleaved, antithrombin (ATx) in the presence of intact antithrombin (AT-III). The inventive method includes a modified ELISA using a detergent to alter the intact AT-III. Cleaved AT-III is generated in human plasma, then an ELISA is performed in the presence of a detergent.

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