Method of detecting phosphatase

Details Method of detecting phosphatase Method of detecting phosphatase

1993-11-23

1996-01-02

Zitomer, Stephanie W.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

435 6, C12Q 168, C12N 916

Patent

active

054807913

ABSTRACT:
An easy operable method of detecting phosphatase which comprises a step for the production of a dye to obtain an azo dye by the reaction of phosphatase in a tissue or cell or on chromosome with a 2'-naphthol AS phosphate, specifically, 3-hydroxy- N-2'-biphenyl-2-naphthalenecarboxamide phosphate ester, followed by the reaction between the dephosphorylated 2'-naphthol AS compound with a diazonium salt; an excitation step for the irradiation of excited light to the azo dye; and a detection step for the detection of fluorescence which is emitted upon irradiation of the excited light. The fluorescence is intense and lasts for a long time.

REFERENCES:
E. J. M. Speel et al., "A Novel Fluorescence Detection Method for in situ Hybridization, Based on the Alkaline Phosphatase-Fast Red Reaction", The Journal of Histochemistry and Cytochemistry, vol. 40, No. 9, pp. 1299-1308 (1992).
The Histochemical Journal, vol. 24, No. 8, Abstracts of the 9th International Congress of Histochemistry and Cytochemistry (1992).
P. Kupcsulik et al, Acta Histochemica, vol. 35, No. 2, (1970) pp. 363-371.
J. Schroeder et al, Bioengineering, vol. 5, No. 3-4 (1989) pp. 24-26.
S. West et al, Analytical Biochemistry, vol. 190, No. 2, 1 Nov. 1990, New York, pp. 254-248.
H. J. Hoeltke et al, Analytical Biochemistry, vol. 207, No. 1, 15 Nov. 1992, New York, pp. 24-31.
West et al., Analytical Biochem. 190:254-258 (1990).
Ziomek et al., J. Histochem. Cytochem. 38(3):437-442 (1990).
Sigma Chem. Co. Catalog (1991) pp. 701-703.

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