Method of detecting nucleic acids or the like

Details Method of detecting nucleic acids or the like Method of detecting nucleic acids or the like

1997-03-28

1998-11-03

Robinson, Douglas W.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 21, 435 681, 435968, 436 86, 436 87, 436 89, 436800, 536 243, 530350, C12Q 168, G01N 3300, C07H 2100, C07K 100

Patent

active

058306668

ABSTRACT:
A method of detecting nucleic acids, proteins, or protein nucleic acid complexes. The method includes binding an enzyme, such as phosphatase, to a specimen of the nucleic acid, protein, or protein nucleic acid complex. The enzyme is then reacted with a fluorescein derivative phosphate ester to obtain a fluorescein derivative phosphate ester hydrolysate. The hydrolysate is then irradiated with excitation light, and the emitted fluorescein is detected.

REFERENCES:
Tang et al., "Synthesis of Fluorescein Magnesium Monophosphate for Determination of Acid Phosphatase," Huaxue Shiji(Chinese), 18(3), 179-180 (1996); Chem. Abstr. 126(5), p. 284, Abstr. No. 56775u (Feb. 3, 1997); only Abstract supplied.
Fujita et al., "Efficient Detection of Membrane-Bound Nucleic Acids Using New Fluorescein Derivative," Chemistry Leters, Issue. No. 12, 1073-1074 (Dec. 1996).

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