Method of detecting nucleic acids

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

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435 911, 536 243, 536 2433, 536 231, 935 1, 935 16, 935 77, 935 76, C12Q 168

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06066455&

ABSTRACT:
Method of detecting a nucleic acid by converting the nucleic acid into a single-stranded nucleic acid under alkaline conditions, wherein at least one detergent from the group of anionic, non-ionic and zwitterionic detergents is present, adding an immobilizable or immobilized capture probe, hybridizing the capture probe with the nucleic acid under immobilization of the nucleic acid via the capture probe and detecting the amount of synthesized, immobilized hybrid.

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