Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1996-03-29
1998-03-17
Zitomer, Stephanie W.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
935 77, 935 78, 536243, 435 65, C12Q 168, C12N 1500, C07H 2104
Patent
active
057285314
ABSTRACT:
A method of detecting nucleic acid utilizes hybridization between the nucleic acid to be detected which is present on an immobilizing support and a DNA probe which is complementary to at least a portion of the desired nucleotide sequence of the nucleic acid. The present DNA probe includes a hapten attached via a linker which a hapten is gibberellin or a gibberellin derivative. After hybridization by reaction between the nucleic acid to be detected and the DNA probe, the unreacted DNA probe is removed, and then labelled anti-hapten antibody is allowed to bind with the DNA probe of the hybrid and the labelling is used to detect the nucleic acid.
Accordingly, stable detecting sensitivity may be achieved regardless of the label mixing ratio or labelling ratio, and thus regardless of the nucleotide sequence composition of the nucleic acid being tested.
REFERENCES:
patent: 5354657 (1994-10-01), Holtke et al.
Matthews et al., Analytical Biochemistry 169: 1-25 (1988).
FEBS Lett., vol. 202, No. 1, pp. 27-31, 1986, J. Eberle, et al., "Monoclonal Antibodies Against GA.sub.13 -Imide Recognize the Endogenous Plant Growth Regulator, GA.sub.4, and Related Gibberellins".
Plant Cell Physiology, vol. 32, No. 4, pp. 505-510, 1991, Masatoshi Nakajima, et al., "Preparation and Validation of an Antisueum Specific for Non-Derivatized Gibberellins".
Asami Osamu
Fujita Satoshi
Kagiyama Naoto
Kondoh Yasumitsu
Momiyama Masayoshi
Aisin Seiki Kabushiki Kaisha
Kabushiki Kaisha Toyota Chuo Kenkyusho
Whisenant Ethan
Zitomer Stephanie W.
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