Method of detecting nucleic acid

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

935 77, 935 78, 536243, 435 65, C12Q 168, C12N 1500, C07H 2104

Patent

active

057285314

ABSTRACT:
A method of detecting nucleic acid utilizes hybridization between the nucleic acid to be detected which is present on an immobilizing support and a DNA probe which is complementary to at least a portion of the desired nucleotide sequence of the nucleic acid. The present DNA probe includes a hapten attached via a linker which a hapten is gibberellin or a gibberellin derivative. After hybridization by reaction between the nucleic acid to be detected and the DNA probe, the unreacted DNA probe is removed, and then labelled anti-hapten antibody is allowed to bind with the DNA probe of the hybrid and the labelling is used to detect the nucleic acid.
Accordingly, stable detecting sensitivity may be achieved regardless of the label mixing ratio or labelling ratio, and thus regardless of the nucleotide sequence composition of the nucleic acid being tested.

REFERENCES:
patent: 5354657 (1994-10-01), Holtke et al.
Matthews et al., Analytical Biochemistry 169: 1-25 (1988).
FEBS Lett., vol. 202, No. 1, pp. 27-31, 1986, J. Eberle, et al., "Monoclonal Antibodies Against GA.sub.13 -Imide Recognize the Endogenous Plant Growth Regulator, GA.sub.4, and Related Gibberellins".
Plant Cell Physiology, vol. 32, No. 4, pp. 505-510, 1991, Masatoshi Nakajima, et al., "Preparation and Validation of an Antisueum Specific for Non-Derivatized Gibberellins".

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Method of detecting nucleic acid does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method of detecting nucleic acid, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method of detecting nucleic acid will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-956469

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.