Method of detecting nitrate esters

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Chemical modification or the reaction product thereof – e.g.,...

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435 793, 435 795, 436815, 5303898, C07K 1300

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active

053670612

ABSTRACT:
A method of detecting the presence and amount of a nitrate ester of a polyol in an unknown comprises preparing an antigen based on such an ester and using that antigen to raise antibodies for use in a biochemical assay for the nitrate ester. A preferred antigen is pentaerythritol trinitrate bonded to a protein such as thyroglobulin or BSA. Antibodies can be raised against this antigen in rabbits or other normally used laboratory animals, and the antibodies are used in, for example, a competitive inhibition enzyme immunosorbent assay (CIEIA).

REFERENCES:
P. Tijssen, Practice and Theory of Enzyme Immunoassays, pp. 249-251, 279-287, Elsevier, N.Y. (1985).
B. Erlanger, Pharmacol. Revs., vol. 25, No. 2 (1973) pp. 271-280.

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