Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2008-07-01
2008-07-01
Switzer, Juliet C. (Department: 1634)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C536S024300, C435S091200
Reexamination Certificate
active
10839202
ABSTRACT:
A method of detecting micrometastasis of tumor cells in a sample obtained from a region of a subject other than the primary focus of the tumor by using a first primer complementary to part of a specific sequence in the RNA from tumor cells and a second primer homologous to part of the specific sequence (either of which additionally has a promoter sequence for an RNA polymerase at the 5′ end), which comprises (1) synthesizing a cDNA by the action of an enzyme having RNA-dependent DNA polymerase activity by using the specific sequence as a template, (2) degrading the RNA strand in the RNA-DNA double strand by an enzyme having a ribonuclease H activity (to give a single-stranded DNA), (3) forming a double-stranded DNA having a promoter sequence which can be transcribed into an RNA homologous or complementary to the specific sequence by using the single-stranded DNA as a template by the action of an enzyme having DNA-dependent DNA polymerase activity, and (4) transcribing the double-stranded DNA into an RNA transcript (which acts as a template in the subsequent cDNA synthesis in the reaction (1)) by the action of an enzyme having RNA polymerase activity and detecting the mRNA.
REFERENCES:
patent: 6057105 (2000-05-01), Hoon et al.
patent: 6329179 (2001-12-01), Kopreski
patent: 7056660 (2006-06-01), Giesing et al.
patent: 2002/0098492 (2002-07-01), Taya
patent: WO 96/29430 (1996-09-01), None
patent: WO 97/18322 (1997-05-01), None
patent: WO 99/32644 (1999-07-01), None
patent: WO 01/18245 (2001-03-01), None
patent: WO 01/49716 (2001-07-01), None
patent: WO 02/12328 (2002-02-01), None
patent: WO 03/085087 (2003-10-01), None
GENBANK Accession M29540 GI: 180222 PRI Nov. 1994.
Deiman, Birgit et al. Characteristics and Applications of Nucleic Acid Sequence Based Amplification. 2002 Moleuclar Biotechnology. vol. 20 pp. 163-179.
A. C. Lambrechts, et al., Breast Cancer Research and Treatment, vol. 56, No. 3, XP-009035704, pp. 219-231, “Comparison of Immunocytochemistry, Reserve Transcriptase Polymerase Chain Reaction, and Nucleic Acid Sequence-Based Amplification for the Detection of Circulating Breast Cancer Cells”, Aug. 1999.
H. Nogi, et al., Breast Cancer, vol. 10, No. 1, XP-009035695, pp. 74-81, “Detection of MUC1 and Keratin 19 mRNAs in the Bone Marrow by Quantitative RT-PCR Predicts the Risk of Distant Metastasis in Breast Cancer Patients”, Jan. 2003.
Schrewe, H. et al., “Cloning of the complete gene for carcinoembryonic antigen: analysis of its promoter indicates a region conveying cell type-specific expression”, Mol. Cell. Biol. 10 (6), 1990, pp. 2738-2748, M59256, Reports, Nucleotide, 2 pages.
Article on the “Preparation and Utilization of Isolated and Purified Oligonucleotides” by Andrew Chin, Mar. 9, 2002 (Paper Copy & CD.
Hayashi Toshinori
Oonaka Satoru
Shaw Amanda
Switzer Juliet C.
Tosoh Corporation
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