Method of detecting human β-defensins

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

Reexamination Certificate

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C435S007920, C435S962000, C436S501000, C436S825000

Reexamination Certificate

active

08076088

ABSTRACT:
A method of detecting β-defensin in a bodily sample from a subject includes reducing the electrostatic interaction between β-defensin and negatively charged moieties in the bodily sample prior to detecting the β-defensin with an antibody or epitope binding fragment thereof.

REFERENCES:
patent: 2007/0093416 (2007-04-01), Igarashi et al.
Ghosh et al., Quantification of Human B-Defensin- and -3 in Body Fluids: Application for Studies of Innate Immunity, Clinical Chemistry 53:4 Apr. 2007, pp. 757-765.
Yin et al., Mouse salivary glands and human B-defensin-2 as a study model for antimicrobial gene therapy: technical considerations, International Journal of Antimicrobial Agents 28, 2006, pp. 352-360.

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