Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving virus or bacteriophage
Patent
1991-04-04
1992-12-15
Nucker, Christine M.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving virus or bacteriophage
435 71, 435 792, 435 23, 435 24, 435974, G01N 3350, C12Q 100
Patent
active
051716623
ABSTRACT:
A method for identifying compounds that inhibit HIV protease is disclosed. A substrate that comprises an HIV protease cleavage site is combined with HIV protease and test compounds. Cleavage of the substrate indicates protease activity and can be detected using antibodies against a cleavage product which do not cross react with uncleaved substrate. A method of detecting the presence of anti-HIV protease antibodies in a sample is also disclosed. A substrate is combined with the sample and HIV protease. Detection of substrate cleavage indicates that the protease is active and that there is an absence of neutralizing anti-HIV protease antibodies.
REFERENCES:
patent: 4918001 (1990-04-01), Kolde
patent: 4952493 (1990-08-01), Kettner et al.
patent: 5011910 (1991-04-01), Marshall et al.
Bu, Ming et al. "Inhibition of Bacterially Expressed HIV Protease Activity Determined by an In Vitro Cleavage Assay with MuLV Pr65.sup.gag ". AIDS Research and Human Retroviruses. 5(3)1989, 259-268.
Evans, D. B., et al., "Substrate Specificity and Inhibitor Structure-Activity Relationships of Recombinant Human Renin: Implications in the in Vivo Evaluation of Renin Inhibitors," (1990), Biotechnol. Appl. Biochem. 12:161-175.
Gottfried, T. D. and H. B. Urnovitz, "HIV-1 Testing: Product Development Strategies," (1990), Tibtech 8:35-40.
D. P. Bolognesi, "Approaches to HIV Vaccine Design," (1990), Tibtech 8:40-45.
Cornette, J. C., et al., "Renin Activity Determination Using Human Plasma as a Substrate," (1987), Analyt. Biochem., 163:93-99.
Sharma, S. K., et al., "A Primate Bioassay for the Determination of Renin Inhibitory Peptides in Serum," (1990), Analyt. Biochem., 186:24-27.
"GammaCoat.TM. [.sup.125 I] Plasma Renin Activity Radioimmunoassay Kit," Baxter Cat. Nos. CA-533-553.
Wondrak, E. M., et al., "A Solid Phase Assay for the Protease of Human Immunodeficiency Virus," (1990), Analyt. Biochem., 188:82-85.
Hansen, J., et al., "Partial Purification and Substrate Analysis of Bacterially Expressed HIV Protease by Means of Monoclonal Antibody," (1988), The EMBO Journal, 6:1785-1791.
Giam, C-Z and I. Boros, "In Vivo and In Vitro Autoprocessing of Human Immunodeficiency Virus Protease Expressed in Escherichia coli," (1988), J. Biol. Chem., 263:14617-14620.
Nutt, R. F., et al., "Chemical Synthesis and Enzymatic Activity of a 99-Residue Peptide With a Sequence Proposed for the Human Immunodeficiency Virus Protease," (1988), Proc. Natl. Acad. Sci. USA, 85:7129-7133.
Krausslich, H-G, et al., "Processing of In-Vitro-Synthesized gag Precursor Proteins of Human Immunodeficiency Virus (HIV) Type 1 by HIV Proteinase Generated in Escherichia coli," (1988), J. of Virology, 62:4393-4397.
Louis, J. M., et al., "Chemical Synthesis and Expression of the HIV-1 Protease Gene in E. Coli," (1989), Biochem. & Biophys. Res. Comm., 159:87-94.
Moore, M. L., et al., "Peptide Substrates and Inhibitors of the HIV-1 Protease," (1989), 159:420-425.
Vlasuk, G. P., et al., "Purification and Characterization of Human Immunodeficiency Virus (HIV) Core Precursor (p55) Expressed in Saccharomyces cerevisiae," (1989), 264:12106-12112.
Blumenstein, J. J., et al., "Synthetic Non-Peptide Inhibitors of HIV Protease," (1989), Biochem. & Biophys. Res. Comm., 163:980-987.
DeLuca Mark
Dubrule Chris
Nucker Christine M.
The Upjohn Company
LandOfFree
Method of detecting HIV protease activity does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method of detecting HIV protease activity, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method of detecting HIV protease activity will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2092129