Method of detecting heparin-induced thrombocytopenia

Details Method of detecting heparin-induced thrombocytopenia Method of detecting heparin-induced thrombocytopenia

1997-02-21

1999-10-26

Feisee, Lila

Chemistry: analytical and immunological testing

For preexisting immune complex or auto-immune disease

436518, 436501, 436808, 436 69, 435 13, 435810, 435975, 521 29, 53038823, G01N 3353

Patent

active

059727187

ABSTRACT:
A method of detecting heparin-induced antibodies to complete a diagnosis of heparin-induced thrombocytopenia HITP is disclosed. In one embodiment, this method comprises binding human platelet factor 4 to a linear, non-glycosaminoglycan polymer carrying negative charges distributed along the polymer chain, wherein the negative charge carried by the polymer is less than 10 .ANG. from the polymer chain. In another embodiment, the negative charge is a strong negative charge. A complex having one or more epitopes recognizable by antibodies generated in a HITP immune response is formed. One then contacts blood plasma or serum from a human patient suspected of having HITP with the complex and analyzes the complex to determine if the HITP-related antibodies are present. In another embodiment of the invention, a kit for diagnosing HITP is disclosed.

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