Method of detecting hematopoietic progenitor cells

Details Method of detecting hematopoietic progenitor cells Method of detecting hematopoietic progenitor cells

1997-03-28

1998-11-03

Degen, Nancy

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving viable micro-organism

435 4, 435 34, 435325, 435366, 435372, 436164, 436172, C12Q 104, C12Q 106, C12N 508, G01N 2176

Patent

active

058307010

ABSTRACT:
Improved method for detecting and/or counting the appearance of hematopoietic progenitor cells which comprises the steps of mixing a blood sample with a reagent capable of detecting immature cells without employing any immunological techniques, obtaining cell information about the thus treated sample using a particle analyzer and constructing a cell distribution profile, delineating a portion of the profile as a zone in which at least one subclass of hematopoietic progenitor cells appear, and counting the cells within the zone. The method is capable of detecting the appearance of hematopoietic progenitor cells in a simple and positive manner.

REFERENCES:
patent: 5413938 (1995-05-01), Tsujino et al.
patent: 5538893 (1996-07-01), Sakata et al.
Atzpodien et al "Human Bone Marrow CFU-GM & BFU-E Localized by Light Scatter Cell Sorting" Exp. Cell Biol. 55 (5) 265-270, 1987.

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