Chemistry: analytical and immunological testing – Cancer
Patent
1996-05-30
1998-10-20
Pyon, Harold Y.
Chemistry: analytical and immunological testing
Cancer
436 63, 436 71, 436161, 436162, 436177, 436103, 436104, 436105, G01N 3350
Patent
active
058245553
ABSTRACT:
The present invention provides a new method for detecting the presence of gynecological carcinomas in a patient. The method, which comprises detecting the presence of lysophosphatidic acid in a plasma sample of the patient, is useful for detecting ovarian carcinoma, cervical carcinoma, endometrial carcinoma, and peritoneal carcinoma. In a preferred embodiment the method comprises: providing a blood specimen from the patient, obtaining a plasma sample for the blood specimen under conditions which minimize the release of lysophosphatidic acid from the platelets in the blood specimen into the plasma, extracting lipids from the plasma, and detecting the presence of lysophosphatidic acid in the lipid extract. A new method for extracting lysophosphatidic acid from a biological fluid is also provided. The method which comprises acidifying the biological fluid and extracting the lysophosphatidic acid from the acidified sample with an organic solvent is useful for extracting greater than 80% of the lysophosphatidic acid in the biological fluid.
REFERENCES:
patent: 4999344 (1991-03-01), Jett-Tilton et al.
patent: 5277917 (1994-01-01), Xu et al.
patent: 5326690 (1994-07-01), Xu et al.
patent: 5595738 (1997-01-01), Pomato
Xu et al. Lysophosphatidic acid, a diagnostic biometer for ovarian and other gynecological cancers. Eighty-eight Annual Meeting of the American Association for Cancer Research, San Diego, California, 1997.
Apr. 12-16, 1997. Proceedings of the American Association for Cancer Research Annual Meeting 38 (o) Abstract.
Imagawa et al. Analysis of the proliferative response to lysophosphatidic acid in primary cultures of mammary epithelium: Difference between normal and tumor cells. Experimental Cell Research 216 (1) 1995 178-186. Abstract, 1995.
Sklar et al. Retinoid Binding Lipoprotein in Neoplastic Cells. Cancer Lett. 22 (1), 1984, 41-48.
Imamura et al. Induction of In-Vitro Tumor cell invasion of cellular monolayers by -lysophosphatidic acid or phospholipase D. Biochem Biophys. Res. Comm. 193 (2) 497-503, 1993.
Frankel et al. "Peptide and lipid growth factors decrease cis-diammine dichloro platinum-induced cell death in human ovarian cancer cells." Clin. Cancer Res. 2 (8), 1307-1313, 1996.
Xu, et al., "Activation of Human Ovarian Cancer Cells: Role of Lipid Factors in Ascitic Fluid" Chapman and Hall Medical, London, Glasgow, Weinheim, New York, Tokyo, Melbourne, and Madras, pp. 121-135 (1994).
Xu, et al., "Effects of Lysophospholipids on Signaling in the Human Jurkat Cell Line" J. Cell. Physiology, 163: 441-450 (1995).
Xu, et al., "Lysophospholipids Activate Ovarian and Breast Cancer Cells" Biochem. J., 309: 933-940.
Xu, et al., "Characterization of an Ovarian Cancer Activating Factor (OCAF) in Ascites from Ovarian Cancer Patients" Clinical Cancer Res., 1: 1223-1232 (1995).
Eichholitz et al., "The Bioactive Phospholipid Lysophosphatidic Acid is Released From Activated Platelets" Biochem. J. 291: 677-680 (1993).
Gerrard et al., "Increased Phosphatidic Acid and Decreased Iysophosphatidic Acid in Response to Thrombin is Associated With Inhibition of Platelet Aggregation" Biochim. Biophys. Acta, 1001: 282-285 (1989).
Jalink et al., "Growth Factor-Like Effects of Lysophosphatidic Acid, a Novel Lipid Mediator" Biochim. Biohphy. Acta 1198: 185-196 (1994).
Moolenaar, "LPA: A Novel Lipid Mediator with Diverse Biological Actions" Trends Cell Biol. 4: 213-218 (1994).
Moolenaar, "Lysophosphatic Acid Signalling" Curr. Opi Cell Biology 7: 203-210 (1995).
Moolenaar, "Lysophosphatidic Acid, a Multifunctional Phospholipid Messenger" J. Biol. Chem. 270: 12949-12952 (1995).
Tigyi et al., "Lysophosphatidic Acid Possesses Dual Action in Cell Proliferation" Proc. Natl. Acad. USA. 91: 1908-1912 (1994).
Watson et al, "Decanoyl Lysophosphatidic Acid Induces Platelet Aggregation Through an Extracellular Action" Biochem. J. 232: 61-66 (1985).
Casey Graham
Xu Yan
Carrillo Sharidan
Pyon Harold Y.
The Cleveland Clinic Foundation
LandOfFree
Method of detecting gynecological carcinomas does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method of detecting gynecological carcinomas, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method of detecting gynecological carcinomas will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-243595