Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism
Patent
1995-02-27
1996-11-05
Elliott, George C.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving viable micro-organism
435 34, 435 6, C12Q 102, C12Q 104, C12Q 168
Patent
active
055716881
ABSTRACT:
A reporter system relating to in vivo expression technology was devised to aid in the identification and study of genes that display temporal or spatial patterns of expression during infection of host tissues. The method of this invention comprises constructing a strain or pool of strains of a microorganism which contains an artificial cointegrate comprising a reporter gene flanked by direct repeats of sequences to which a resolvase enzyme binds, thus catalyzing excision of the reporter gene, and further contains a coding sequence under the control of a promoter sequence which encodes transcripts, the expression of which are easily monitored in vitro and which result in a permanent genetic change, excision of the reporter gene, that is heritable and easily detectable subsequent to induction of the synthetic operon.
REFERENCES:
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Osbourn et al., "Identification of Plant-Induced Genes of the Bacterial Pathogen Xanthomonas campestris Pathovar campestris Using a Promoter-Probe Plasmid", EMBO Journal, vol. 6, No. 1, pp. 23-28.
Camilli Andrew
Mekalanos John J.
Elliott George C.
Petersen Steven C.
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