Method of detecting fluorescent materials and apparatus for thei

Details Method of detecting fluorescent materials and apparatus for thei Method of detecting fluorescent materials and apparatus for thei

1981-08-12

1983-12-06

Marcus, Michael S.

Chemistry: analytical and immunological testing

Peptide, protein or amino acid

Amino acid or sequencing procedure

436 93, 436 96, 436 98, 436103, 436140, 436161, 436172, 422 52, G01N 3108, G01N 2176

Patent

active

044194528

ABSTRACT:
A method for detecting fluorescent materials in liquid chromatography. Fluorescent materials are excited by chemical reaction with chemical reagents to yield light (chemiluminescence) and detected at high sensitivity. The detection apparatus is composed of a mixer for mixing a solution containing separated fluorescent materials and solutions of chemical reagents to afford chemiluminescence, a flow cell and a light receptive detection means for detecting the light thus produced.

REFERENCES:
patent: 3915648 (1975-10-01), Stein
patent: 4058365 (1977-11-01), Krogh
patent: 4076645 (1978-02-01), Vega
patent: 4220450 (1980-09-01), Maggio
Curtis et al., J. of Chrom., 134 (1977), 343-350.

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