Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1996-08-27
1999-05-18
Saunders, David
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
436 64, 436 86, 436813, 530413, 530827, 530832, G01N33/574;33/68
Patent
active
059050268
ABSTRACT:
The present disclosure teaches that the proteins encoded by the BRCA1 and BRCA2 genes are found in the milk fat globule membranes from humans and cows. Therefore, BRCA1 and BRCA2 proteins can be isolated from milk produced by lactating animals. The level of expression of BRCA1 or BRCA2 can be determined by sampling the levels of BCRA1 or BRCA2 proteins found in the MFGM of lactating animals. The present invention also includes a method of determining the likelihood that a woman will develop breast cancer by measuring the amount of the BRCA1 or JBRCA2 expression during lactation. The detection of expression of BRCA1 or BRCA2 can be accomplished with an antibody raised specifically against BRCA1 or BRCA2 proteins, by isolating BCRA1 or BRCA2 from the milk fat globule membranes or by detecting activity of BRCA1 or BRCA2. The level of BRCA1 or BRCA2 is compared to a reference scale of propensity for breast cancer development correlated to normally active BRCA1 or BRCA2 levels. BRCA1, BRCA2, fatty acid binding proteins and phosphorylated proteins are found in the MFGM and the MFGM can be isolated from milk and provided in a form suitable for oral consumption, i.e. a tablet or capsule of separated milk fat globule membranes, a food additive, or a fortified dairy commodity.
REFERENCES:
Miki,Y. et al, (1994) A Stong Candidate for the Breast and Ovarian Cancer Susceptibility Gene BRCA1, Science 266, pp. 66-71.
Futreal, A et al, (1994) BRCA1 Mutations In Primary Breast and Ovarian Carcinomas, Science 266, pp. 120-126.
Tavtigian, S.V. et al, (1996) The Complete BRCA2 gene and mutations in chromosomes 13q-linked Kindreds, Nature Genetics 12, pp. 333-337.
Chen, Y et al (1995) Aberrant Subcellular Localization of BRCA1 In Breast Cancer, Science 270, pp. 789-791.
Scully, R. et al (1996), location of BRCA1 In Human Breast and Ovarian Cells, Science 272, pp. 123-124.
Thompson M.E. et al, (1996) Decreased Expression of BRCA1 Accelerates Growth and Is Often present During Sporadic Breast Cancer Progression, Nature Genetics 9, 444-450.
Jensen, R. et al, (1996) BRCA1 is Secreted And Exhibits Properties of a Granin, Nature Genetics 12, 303-308.
Spitsberg, V.S. et al (1996) Identification of Immunoreactive BRCA1 Protein in Milk and Mammary Tissue ASBMB/ASIP/AAI Joint Meeting With ASBMB Satellite Meetings. Program addendum and Late-breaking abstracts, Ernest N. Morial Convention Center, New Orleans, LA, Jun. 1-6.
Spitsberg, V.L. et al, (1995) Phosphorylation of Bovine and Human Milk Fat Globule Membrane (MFGM) and Bovine Skim Milk Membrane (SSM) proteins Effect Metals Inhibitors and Triton X-100, The FASER j. ((3)#495.
Spitsberg, V.L. et al, (1996) Is the MFGM Associated Protein Butyriphilin (Bph) a Kinase? The FASEB j. 10 , A684, #3946.
Spitsberg, V.L. et al, (1995) Association and Coexpression of Fatty Acid Binding Protein and Glycoprotein CD36 In The Bovine Mammary Gland, Eur J. Biochem. 230, 872-878.
LeGendre, N et al, (1989) Purification of Proteins and Peptides by SDS-PAGE, In Practical Guide to Protein and Peptide Purification for Microsequencing, Ed. D.T. Matsudaria, AP, pp. 49-69.
Gorewit Ronald C.
Spitsberg Vitaly L.
Cornell Research Foundation Inc.
Saunders David
LandOfFree
Method of detecting expression of and isolating the protein enco does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method of detecting expression of and isolating the protein enco, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method of detecting expression of and isolating the protein enco will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1759305